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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in several tumors, which leads to chromosome instability, and therefore might contribute to tumorigenesis. This protein was uncovered to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a significant position in marketing mobile-cycle development within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two further splice variants were described but in depth nucleotide sequence details is not really nevertheless accessible.
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Blocking peptides are peptides that bind precisely towards the focus on antibody and block antibody binding. These peptide usually is made up of the epitope regarded through the antibody.
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This manual outlines further feasible optimization of cellular and immunoassay parameters to be certain the best possible success are...
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This guide outlines additional attainable optimization of cellular and immunoassay parameters to make certain the absolute best effects are...
Antibodies sure to the blocking peptide no more bind on the epitope to the focus on protein. By comparing the staining from your blocked antibody compared to the antibody alone, one can see which staining is unique.
Concentrate on Information Cyclin E belongs to the highly conserved cyclin spouse and children, whose users are characterised by a extraordinary periodicity in protein abundance with the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation styles which add for the temporal coordination of each mitotic party. Cyclin E varieties a complex with and capabilities for a regulatory subunit of CDK2, whose action is necessary for mobile cycle G1/S transition. This protein accumulates for the G1-S stage boundary and is degraded as cells development by means of S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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